Anbio Biotechnology

Our Corporate History and Structure The following information illustrates the corporate structure of Anbio Biotechnology and its subsidiaries as of the date of this annual report. Anbio Biotechnology was incorporated under the laws of the Cayman Islands as an exempted company with limited liability on July 27, 2021. 15 Table of Contents Anbio Australia was incorporated under the laws of Australia on October 6, 2021, which became a wholly owned subsidiary of Anbio on February 21, 2022. Anbio Australia has no operation as of the date of this annual report. Anbio Samoa was incorporated under the laws of the Independent State of Samoa on February 25, 2025, as a wholly owned subsidiary of PharVac BVI to design and supply IVD products. PharVac BVI was incorporated under the laws of the British Virgin Islands on April 13, 2022, as a wholly owned subsidiary of Anbio. PharVac BVI is an investment holding company and has business no operation as of the date of this annual report. PharVac USA was incorporated under the law of Delaware on January 18, 2023, as a wholly owned subsidiary of PharVac BVI. PharVac USA has no operation as of the date of this annual report. LoviWell BVI was incorporated under the law of the British Virgin Islands on February 22, 2023, as a wholly owned subsidiary of Anbio. LoviWell BVI has no operation as of the date of this annual report. LoviWell USA was incorporated under the law of Delaware on March 28, 2023, as a wholly owned subsidiary of LoviWell BVI. LoviWell has no operation as of the date of this annual report.